Advances In Biochemical Engineering Biotechnology Plant by J.-J. Zhong

By J.-J. Zhong

The hot achievements in engineering reviews on plant cellphone cultures are reviewed, integrated are the gasoline focus results and bioprocess integration for the improved productiveness of plant secondary metabolites. The metabolic engineering of plant secondary metabolite pathways and recombinant protein creation from genetically converted plant cells are brought. Large-scale plant micropropagation through somatic embryogenesis and furry roots is mentioned for effective propagation of desease-free, genetically uniform and big quantities of vegetation in vitro in significant quantities. Characterization and alertness of furry plant roots endowed with photosynthetic features is additionally coated during this unique quantity.

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This value –1 was approximately 3 ¥ 10–7 mol g–1 dw s . Nevertheless, the value of Hallsby’s esti–10 –1 –1 mate of uptake of oxygen, 3 ¥ 10 g–1 dw mol s for N. tabacum, will be used [32, 33]. Hulst’s observation that specific consumption was similar for suspended and immobilized cells is assumed to apply [31]. During most of our studies with calcium alginate immobilized N. 04 g (dry weight) of cells; of course, this value varied from test to test. It is reasonable to assume that the feed medium was saturated in O2 .

2001 10:14 Uhr Seite 51 (Schwarz/Process Black Bogen) Gas Concentration Effects on Secondary Metabolite Production by Plant Cell Cultures 51 thought to carry the signal from the elicited plant to the surrounding unelicited plants. Elicitation of plant cell suspension cultures is normally carried out by addition of a solution of the elicitor. Indeed, for investigators without the means for mixing gases and supplying the mixture to the plant cell culture, application of the plant hormone ethylene is normally conducted by addition of a dilute ethephon solution to the plant suspension culture.

3 4 Conclusions . . . . . . . . . . . . . . . . 55 References . . . . . . . . . . . . . . . . . . 59 1 Introduction Plants provide a wide variety of biochemicals useful to humanity. Their uses include medicinal compounds, flavors, fragrances and agricultural chemicals. A number of investigators have studied the use of plant cells in culture, rather than whole plants, as sources of some of the more valuable organic compounds. Before such processes can become a viable manufacturing option, a great deal more must be learned about the optimum conditions for growth and productivity of cells in culture.

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